Search results for "Replication factor C"

showing 3 items of 3 documents

The DNA-binding subunit p140 of replication factor C is upregulated in cycling cells and associates with G 1 phase cell cycle regulatory proteins

1999

The DNA-binding subunit of replication factor C (RFCp140) plays an important role in both DNA replication and DNA repair. The mechanisms regulating activation of RFCp140 thereby controlling replication and cellular proliferation are largely unknown. We analyzed protein expression of RFCp140 during cell cycle progression and investigated the association of RFCp140 with cell cycle regulatory proteins in cell lines of various tissue origin and in primary hematopoietic cells. Western and Northern blot analyses of RFCp140 from synchronized cells showed downregulation of RFCp140 when cells enter a G0-like quiescent state and upregulation of RFCp140 in cycling cells. Translocation from the cytopla…

CytoplasmSaccharomyces cerevisiae ProteinsT-LymphocytesCyclin ACell Cycle ProteinsEukaryotic DNA replicationCell LineMinor Histocompatibility AntigensDNA replication factor CDT1MiceReplication factor CControl of chromosome duplicationDrug DiscoveryAnimalsHumansReplication Protein CGenetics (clinical)Cell NucleusHomeodomain ProteinsbiologyG1 PhaseS-phase-promoting factor3T3 CellsCell cycleMolecular biologyUp-RegulationCell biologyDNA-Binding ProteinsRepressor ProteinsProto-Oncogene Proteins c-bcl-2biology.proteinMolecular MedicineOrigin recognition complexJournal of Molecular Medicine
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Enhancement of hepatitis C virus RNA replication by cell culture-adaptive mutations.

2001

ABSTRACT Studies of the Hepatitis C virus (HCV) replication cycle have been made possible with the development of subgenomic selectable RNAs that replicate autonomously in cultured cells. In these replicons the region encoding the HCV structural proteins was replaced by the neomycin phosphotransferase gene, allowing the selection of transfected cells that support high-level replication of these RNAs. Subsequent analyses revealed that, within selected cells, HCV RNAs had acquired adaptive mutations that increased the efficiency of colony formation by an unknown mechanism. Using a panel of replicons that differed in their degrees of cell culture adaptation, in this study we show that adaptive…

virusesImmunologyCell Culture TechniquesRNA-dependent RNA polymeraseReplicationHepacivirusBiologyViral Nonstructural ProteinsOrigin of replicationVirus ReplicationMicrobiologyReplication factor CControl of chromosome duplicationGenes ReporterVirologyTumor Cells CulturedHumansRepliconLuciferasesGeneRNAVirologyAdaptation PhysiologicalViral replicationInsect ScienceMutationRNA ViralRepliconJournal of virology
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Viral and cellular determinants of hepatitis C virus RNA replication in cell culture.

2003

Studies on the replication of hepatitis C virus (HCV) have been facilitated by the development of selectable subgenomic replicons replicating in the human hepatoma cell line Huh-7 at a surprisingly high level. Analysis of the replicon population in selected cells revealed the occurrence of cell culture-adaptive mutations that enhance RNA replication substantially. To gain a better understanding of HCV cell culture adaptation, we characterized conserved mutations identified by sequence analysis of 26 independent replicon cell clones for their effect on RNA replication. Mutations enhancing replication were found in nearly every nonstructural (NS) protein, and they could be subdivided into at …

virusesImmunologyCell Culture TechniquesReplicationRNA-dependent RNA polymeraseEukaryotic DNA replicationHepacivirusViral Nonstructural ProteinsBiologyVirus ReplicationOrigin of replicationMicrobiologyReplication factor CControl of chromosome duplicationVirologyTumor Cells Cultured[SDV.BBM] Life Sciences [q-bio]/Biochemistry Molecular BiologyHumansRepliconVirologyAmino Acid SubstitutionViral replicationInsect ScienceRNA ViralOrigin recognition complexRepliconRibosomes
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